UNAIDS’ 2013 modelled estimates that the HIV prevalence in the urban areas of countries in sub-Saharan Africa is twice the national HIV prevalence elsewhere in the countries were multiplied by the population sizes of cities to identify a preliminary list of 300 cities in which the largest number of people living with HIV were likely to reside. For each of the 300 top-ranked cities, a comprehensive review of all available data sources was conducted to refine the modelled estimates and to produce a final estimate of the total number of people living with HIV in each city for the top-ranked 200 cities.